Fig. 1

A comparison of different bioinformatics tool performances at both the species level and genera level using the NIBSC Gut-Mix-RR and Gut-HiLo-RRs. a Relative abundance of each species in the Gut-Mix-RR as calculated by five different metagenomic taxonomic profiling tools in comparison to the known composition of the reagent. b Relative abundance of each species in the Gut-HiLo-RR as calculated by five different metagenomic taxonomic profiling tools in comparison to the known composition of the reagent. c Relative abundance of each genera in the Gut-Mix-RR as calculated by five different metagenomic taxonomic profiling tools and two 16S rRNA taxonomic profiling pipelines in comparison to the known composition of the reagent. d Relative abundance of each genera in the Gut-HiLo-RR as calculated by five different metagenomic taxonomic profiling tools and two 16S rRNA taxonomic profiling pipelines in comparison to the known composition of the reagent. e Reporting measures for pipeline performance for calculating species as evaluated using the Gut-Mix-RR. f Reporting measures for pipeline performance for calculating species as evaluated using the Gut-HiLo-RR. g Reporting measures for pipeline performance for calculating genera as evaluated using the Gut-Mix-RR. h Reporting measures for pipeline performance for calculating genera as evaluated using the Gut-HiLo-RR. Mp MetaPhlAn2, Kj Kaiju, Kr Kraken, Br Bracken, Cn Centrifuge, Sens sensitivity, FPRA false positive relative abundance, Div diversity, Sim similarity