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Fig. 5 | Microbiome

Fig. 5

From: Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer

Fig. 5

Effect of H. hathewayi on colonic epithelial cell proliferation in germ-free and AOM-injected conventional mice. a Immunohistochemistry showing Ki-67 positive cells in the colon of germ-free mice at 20 weeks after gavaging with H. hathewayi, and the relative proportion of Ki-67 positive cells. Scale bar: 50 μm. b Immunohistochemistry showing Ki-67 positive cells in the colon of AOM-injected, microbiota-depleted convectional mice at 6 weeks after bacterial gavage, and the relative proportion of Ki-67 positive cells. Scale bar: 100 μm. C Western blots showed that H. hathewayi increased the colonic protein expression of DNMT1 and DNMT3A in germ-free mice. d Western blots showed that F. nucleatum and H. hathewayi increased colonic protein expression of DNMT1 and DNMT3A in conventional mice. Each lane in c and d corresponds to a colonic sample harvested from an individual mouse. *P < 0.05; **P < 0.01 significantly different between the indicated groups

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