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Fig. 4 | Microbiome

Fig. 4

From: Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer

Fig. 4

In vitro effects of F. nucleatum and H. hathewayi on DNMT expression and activity. aF. nucleatum and H. hathewayi co-incubation increased the global DNA methylation (5-mC) levels in NCM460, HCT116, and HT29 cell lines. bc RT-qPCR and Western blots confirmed that the mRNA and protein expression of DNMTs were positively regulated by F. nucleatum and H. hathewayi. d Nuclear DNMT3A activity was induced by F. nucleatum and H. hathewayi. Results in a to d show the average responses obtained from 3 independent experiments (n = 3 in each experiment). *P < 0.05; **P < 0.01;*** P < 0.001; significantly different between the indicated groups

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