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Fig. 7 | Microbiome

Fig. 7

From: Gut microbiota-derived indole 3-propionic acid protects against radiation toxicity via retaining acyl-CoA-binding protein

Fig. 7

ACBP contributes to the protective function of IPA toward irradiation via PXR. a The presence of gene expression after injection in various organs, including the liver, heart, lung, colon, small intestine, and tongue, as confirmed by bioluminescent imaging. b, c The expression level of ACBP (b) and PXR (c) was examined in the small intestine tissues by qRT-PCR. Significant differences are indicated: *P < 0.05 and ***P < 0.005; Mean ± SEM. Student’s t test, n = 10 per group. d Body weights were compared among four group mice after 12Gy TAI, n = 18 per group; *P < 0.05, **P < 0.01, and ***P < 0.005 represent TAI + sh-ACBP group compared with TAI + IPA group; #P < 0.05, ##P < 0.01, and ###P < 0.005 represent TAI + sh-PXR group compared with TAI + IPA group; Student’s t test. e, f Photographs (e) and length (f) of dissected colon from mice in the four groups, the colon tissues were obtained at day 21 after 12 Gy TAI. Mean ± SEM. Significant differences between each two cohorts are indicated: *P < 0.05 and ***P < 0.005; Student’s t test, n = 5 for TAI group, n = 6 for TAI + IPA group, n = 10 for TAI + sh-ACBP group, n = 9 for TAI + sh-PXR group. g, h, j The expression levels of IL-6 (g), Nrf2 (h), and Glut1 (j) were examined in the small intestine tissues by qRT-PCR. The small intestine tissues were obtained at day 21 after 12 Gy TAI. Mean ± SEM. Significant differences between each two cohorts are indicated: *P < 0.05, **P < 0.01, and ***P < 0.005; Student’s t test, n = 6 for control group, n = 6 for TAI group, n = 6 for TAI + IPA group, n = 10 for TAI + sh-ACBP group, n = 9 for TAI + sh-PXR group. i The morphology of the small intestine was shown by H&E (×100 magnification; Scale bar: 100 μm) and PAS (×1000 magnification; Scale bar: 50 μm) staining. The small intestine tissues were obtained at day 21 after 12 Gy TAI. The arrows point to the goblet cells. k The FITC-dextran in PB was assessed at day 21 after 12 Gy TAI. Mean ± SEM. Significant differences between each two cohorts are indicated: **P < 0.01 and ***P < 0.005; Student’s t test, n = 6 for control group, n = 6 for TAI group, n = 6 for TAI + IPA group, n = 10 for TAI + sh-ACBP group, n = 9 for TAI + sh-PXR group

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