Fig. 6

IPA replenishment reprograms small intestinal protein expression profile following TAI challenge. a Volcano plots of identified different proteins from the small intestine of mice with or without IPA treatment. In the volcano plots, each point represented a protein. b–d Bioinformatics analysis of different proteins in small intestine of mice in IPA gavage group compared to the TAI group through gene ontology (GO) in biological process (b), cellular component (c), and molecular function (d). Information on the number of involved proteins in a term is shown on the x-axis. e Hierarchical cluster analysis for the different proteins in the small intestine of mice in IPA gavage group compared to TAI group. f The expression level of ACBP was examined in small intestine tissues from male mice by qRT-PCR. Mean ± SEM. Significant between each two cohort differences are indicated: *P < 0.05; Student’s t test, n = 18 per group. g The relative level of ACBP were measured at the time of 0, 0.5, 1, 1.5, and 2 h with (or without) 4 Gy irradiation after IPA treatment (37.8 μg/mL) by qRT-PCR. Mean ± SEM. Significant differences between each two cohorts are indicated: ***P < 0.005; Student’s t test. h The mRNA levels of ACBP were examined in HIEC-6 and MODE-K cells which included control, 4 Gy irradiation, and 4 Gy irradiation with IPA supplement. i–k The effects of IPA (37.8 μg/mL) on the proliferation of ACBP siRNA-treated HIEC-6 cells were assessed by cloning formation (i, j) and CCK-8 assays (k), respectively. Mean ± SEM. Significant differences between each two cohorts are indicated: *P < 0.05, **P < 0.01, and ***P < 0.005; Student’s t test. l A model showed the predicted binding site for PXR at 678–668 nt and 488–478 nt of ACBP mRNA promoter named PGL3-ACBP-1 and PGL3-ACBP-2. m The effect of PXR and IPA on PGL3-ACBP-1 reporter was measured by luciferase reporter gene assays in HIEC-6 cells. Mean ± SEM. Significant differences between each two cohorts are indicated: **P < 0.01; Student’s t test. n The expression of ACBP was examined by qRT-PCR after transfection of HIEC-6 cells with si-PXR and (or) treated with IPA (37.8 μg/mL)