Fig. 2

Oral gavage of IPA ameliorates TBI-associated hematopoietic system injury. a, b Photographs (a) and weight (b) of dissected thymuses from mice in the three groups, the thymuses were obtained at day 15 after 4 Gy TBI. Mean ± SEM. Significant differences between each two cohorts are indicated: ***P < 0.005; Student’s t test, n = 12 per group. c, d Photographs (c) and weight (d) of the dissected spleens from mice in the three groups, the spleens were obtained at day 15 after 4 Gy TBI. Significant differences between each two cohorts are indicated: **P < 0.01 and ***P < 0.005; Student’s t test, n = 12 per group. e, f White blood cell (WBC) counts (e) and percentage of lymphocytes (LY%) (f) in PB were measured at day 15 after 4 Gy TBI. The data were presented as means ± SEM (n = 12 per group). Significant differences between each two cohorts are indicated: *P < 0.05 and ***P < 0.005; Student’s t test. g, h The content of IL-6 (g) and MDA (h) in PB were examined. Mean ± SEM. Significant differences between each two cohorts are indicated: **P < 0.01 and ***P < 0.005; Student’s t test, n = 6 for control group; n = 11 for TBI group; n = 12 for TBI + IPA group. i–k Representative FACS plots of HSCs, HPCs. The percentage of hematopoietic progenitor cells (HPCs) and HSC cells in lineage-negative cells were analyzed at day 15 after 4 Gy TBI. The data were presented as means ± SEM (n = 6 per group). Significant differences between each two cohorts are indicated: **P < 0.01 and ***P < 0.005; Student’s t test