Table 1 Culture conditions for isolating organisms
From: Isolation and characterization of diverse microbial representatives from the human skin microbiome
Target | Media | Temperature | Special conditions | Leading number in isolate ID 1 | Research participant(s) |
|---|---|---|---|---|---|
Aerobes | Blood agar (Hardy Diagnostics) | Room temp. | Aerobic | 0 | All |
Aerobes | Blood agar (Hardy Diagnostics) | 37 °C | Aerobic | 1 | 60, 63, 66, 70 |
Anaerobes | Blood agar (Hardy Diagnostics) | 37 °C | Anaerobic3 | 2 | 60, 63, 66, 70 |
Slow growers | R2A (BD Biosciences) | Room temp. | Aerobic | 4 | 63, 66, 70 |
Lactobacilli | MRS (Difco) | Room temp. | Aerobic | 6 | 63, 66, 70 |
Fungi | PDA (BD Biosciences) | Room temp. | Aerobic | 5 | 63, 66, 70 |
Spores | Blood agar (Hardy Diagnostics) | Room temp. | 4 h incubation in 70% EtOH, aerobic | 7 | 63, 66, 70 |
Anaerobic spores | Blood agar (Hardy Diagnostics) | 37 °C | 4 h incubation in 70% EtOH, anaerobic3 | 8 | 66, 70 |
Capnophiles | Blood agar (Hardy Diagnostics) | 37 °C | Aerobic + 5% CO2 | 3 | 60 |
Gram-negative | MacConkey | Room temp. | Aerobic, dark | 6 | 70 |
Halophiles | Medium for halophilic bacteria2 | Room temp. | 10% NaCl, aerobic | 3 | 70 |
Anaerobic halophiles | Medium for halophilic bacteria2 | 37 °C | 10% NaCl, anaerobic3 | 9 | 70 |
Deep skin aerobes | Blood agar (Hardy Diagnostics) | 37 °C | Tape strips #5 and #10, aerobic | TS5, TS10 | 70 |
Deep skin anaerobes | Blood agar (Hardy Diagnostics) | 37 °C | Tape strips #5 and #10, anaerobic3 | TS5a, TS10a | 70 |