Fig. 4

Comparison of salivary microbiome based on flight status at the individual level. NMDS ordination, with Euclidean distances, was generated at the ASV level of centered log ratio (clr) data for each individual. Each dot on the graph represents a sample, with the different colors representing whether the sample was collected pre-flight (red), in-flight (blue), or post flight (green). The closer the dots are to each other on the graph, the more similar the samples are in microbiome composition. The subjects are labeled in the top left end corner of each box and an asterisk (*) beside the subject number indicates microbiome differences based on flight status. Differences are based on ellipses drawn around samples based on a 99% confidence interval. Samples that belong to the same flight status and in the same ellipse, and which do not overlap with other ellipses, are considered distinct groups, thereby having different microbiome profiles