Impact of plants on the diversity and activity of methylotrophs in soil

Microbiome

Table 1 Number of OTUs in sequenced mxaF and xoxF amplicons produced from DNA extracted from soil

Gene	Soil environment	Sequencing platform	Number of OTUs
xoxF1	Unplanted	Roche 454	4
xoxF2	Unplanted	Sanger	1
xoxF3	Unplanted	Sanger	6
xoxF5	Unplanted	Roche 454	13
xoxF5	Pea rhizosphere	Roche 454	19
xoxF5	Wheat rhizosphere	Roche 454	14
mxaF	Unplanted	Roche 454	4
mxaF	Pea rhizosphere	Roche 454	4

Amplicons were produced from DNA samples from unplanted soil, pea rhizosphere soil and wheat rhizosphere soil and analysed by either 454 amplicon sequencing or Sanger sequencing. OTUs were produced using an 80% identity clustering threshold

ISSN: 2049-2618