Fig. 1

Phylogeny of mdh2 sequences retrieved from pea plant rhizosphere compared to mdh2 from methylotrophic bacteria. Sequenced amplicons obtained from environmental DNA and DNA from cultures of methylotrophic bacteria are labelled as “Environmental Sequence” and “Isolate PCR” respectively. Full reference gene sequences were selected from the NCBI nucleotide database. The tree was drawn in Mega7 [30] using the neighbour-joining method. Scale bar indicates 0.02 substitutions per site. Only bootstrap values ≥ 50% (based on 500 replicates) are labelled at branch points. There were a total of 164 amino acid residues in the final dataset