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Fig. 2 | Microbiome

Fig. 2

From: Root ethylene mediates rhizosphere microbial community reconstruction when chemically detecting cyanide produced by neighbouring plants

Fig. 2

Effects of soil cyanide on root ethylene synthesis and release. a The 1-aminocyclopropane-1-carboxylic acid (ACC, ethylene precursor) concentration in aboveground and belowground peanut tissues in different treatments. b Cyanide concentration in cassava and peanut rhizosphere soils and corresponding bulk soils. c Cyanide concentration in peanut roots. d Ethylene (ET) production in peanut roots in different treatments. e qRT-PCR detection of ACS gene (AhACS1 and AhACS2) expression in peanut roots in different treatments. f qRT-PCR detection of ACO gene (AhACO1 and AhACO2) expression in peanut root in different treatments. Treatment I (cassava monocropping), Cms and CBms represent cassava rhizosphere and bulk soil, respectively. Treatment II (cassava and peanut intercropping), Cis, PBis and Pis represent soil from the cassava rhizosphere, the bulk soil and peanut rhizosphere; Pip represents peanut plants from treatment II. Treatment III (peanut monocropping), Pms and PBms represent soil from the peanut rhizosphere and bulk; Pmp represents peanuts from treatment III. Treatment IV (peanut monocropping with exogenous CN− addition), CNp represents peanuts from treatment IV. Control (peanut with water addition), Cp represents peanuts from control. Data of soil and plant cyanide are mean values + SD for triplicate; data of plant ACC concentration, ET production and gene expression are values + SD for 9 replicates. Error bars with different letters indicate a significant difference according to one-way analysis of variance (ANOVA) with Tukey’s HSD test (P < 0.05)

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