Fig. 4

Sequencing accuracy across a range of densities. a Scatter plot showing the Bray-Curtis dissimilarity between the expected community composition and all input densities. Mock community 1 is shown on the left panel, mock community 2 is shown on the middle panel, and mock community 3 on the right panel. b. Plot of the relative abundance of members from the 3 mock communities (solid blue line) in comparison with the cycle threshold observed in the 16S qPCR (solid red line). ml = millilitre