Fig. 2
From: Commensal-derived metabolites govern Vibrio cholerae pathogenesis in host intestine
Germ-free mice transplanted with feces of CL-treated mice are susceptible to V. cholerae infection. a Schematic diagram of the experimental procedure. Germ-free mice (C57BL/6, 8~9 weeks old, n = 6 per group) received fecal suspensions (50 μL) derived from PBS- or CL-treated SPF mice. Following 3 days of stabilization, transplanted mice were challenged with V. cholerae infection (5 × 108 CFU) for 24 h. b The SI and cecum were removed from each mouse for tissue homogenization. Fresh fecal matter was collected to prepare fecal suspensions. Aliquots of lysates or suspensions were serially diluted to count CFU of V. cholerae on LB agar supplemented with 200 μg/mL SM. Values are displayed on a log scale as mean ± SEM for each group. *P < 0.05 versus bacterial CFU detected in the control group. **P < 0.001 versus bacterial CFUs detected in the control group. c In a separate set of experiments (n = 3 per group), small intestines were extracted for tissue homogenization, and aliquots of homogenates were assessed for detection of CT by ELISA. *P < 0.01 versus CT level in the control group