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Table 1 Differences between expected and observed genus abundances in the mock communities (Nshotgun = 4, Namplicon = 3) via a one-sample t test (two-sided) of relative abundances (P values are adjusted via Hommel procedure)

From: Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms

 

Shotgun

Amplicon

Members mock community

MEGAN

V1 V2 one-step

V3 V4 one-step

V1 V2 two-step

V3 V4 two-step

Staphylococcus

0.00002

0.52446

0.09200

0.03994

0.21564

Listeria

0.00395

0.34964

0.53267

0.03003

0.00545

Bacillus

0.00006

0.21420

0.02818

0.29671

0.30589

Pseudomonas

0.13668

0.36721

0.05776

0.38147

0.59037

Escherichia/Shigellaa

NA

0.00462

0.45612

0.00237

0.59037

Shigella a

4.6372 × 10−10

NA

NA

NA

NA

Escherichia a

0.00001

NA

NA

NA

NA

Enterobacteriaceae a

NA

0.87898

0.00004

0.19274

0.00055

Salmonella

3.8092 × 10−6

0.34964

0.05838

0.09712

0.08851

Lactobacillus

0.00297

0.87898

0.53267

0.38147

0.59037

Enterococcus

0.00012

0.04816

0.03746

0.01159

0.00954

  1. aEscherichia/Shigella relatives counted as equivalent