Fig. 1From: Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganismsAverage community composition of bacteria (a) and fungi (b) in the mock community samples sequenced via metagenomic shotgun and 16S rRNA gene amplicon techniques (amplicon: V1 V2, V3 V4, one-step, two-step; shotgun: MEGAN based classification (short reads)). c Bacterial genus-level alpha diversity estimates in comparison to the expected community value. d Principle coordinate analysis of the Bray-Curtis distance between methods and the expected community. Ellipses represent standard deviations of points within the respective groups. Sample sizes for the different approaches are Nshotgun = 4, NV1V2-one-step = 3, NV1V2-two-step = 3, NV3V4-one-step = 3, and NV1V2-two-step = 3Back to article page