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Fig. 6 | Microbiome

Fig. 6

From: Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps

Fig. 6

a Gene clusters encoding for the two types of lanthanide-dependent methanol dehydrogenases XoxF3 and XoxF5 in Methylocella sp. PC1 and PC4. Names of the genes are given above. Another copy of xoxF5 is also present in the genomes of Methylocella PC1 and PC4, but only as a singleton gene (see Additional file 1: Figure S4). b PCR amplification showing the absence of mxaF in Methylocella sp. PC1 (lane 1) and Methylocella sp. PC4 (lane 2) along with positive control Methylocella silvestris BL2 (lane 3) and no template control (lane 4). Lane M represents 1 kb DNA ladder. c Growth of Methylocella sp. PC4 with (red) and without (blue) lanthanum. Cultures were grown in DNMS medium with methanol (20 mM) as the only source of carbon and energy. Each point shows the average of duplicate cultures with error bars (invisible if smaller than symbol size) showing the standard errors. A control without any carbon substrate showing no growth of cells (green) was also performed

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