Fig. 5
From: Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps
a The soluble methane monooxygenase genes in Methylocella sp. PC1 and PC4 isolates compared with their homologues in Methylocella silvestris BL2. Names of the genes are given above, and amino acid identities to their homologous proteins in Methylocella silvestris BL2 are shown in brackets. b Phylogenetic analysis of MmoX (alpha subunit of soluble methane monooxygenase) from new Methylocella isolates (bold red) along with MmoX from other known Methylocella (bold black) strains based on the derived amino acid sequences. Protein names and accession numbers for the sequences are given in brackets. Amino acid sequences were aligned using Mega 7.0 and the optimal tree (drawn to scale, with branch lengths measured in the number of substitutions per site) with the sum of branch length = 0.53 is shown where the evolutionary history was inferred using the neighbour-joining method (392 positions in the final dataset). The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) ranged from 59 to 100