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Table 5 PCR-based screening of nitrogen cycling genes in 10 sponge species from 2 locations, showing the number of samples testing positive (left value) and the total number of samples tested (right value) for 4 functional genes. Amplifications for amoB and nifH genes were repeated in triplicate for each sample

From: Testing the relationship between microbiome composition and flux of carbon and nutrients in Caribbean coral reef sponges

Category Species Location Function gene PCR assays
amoA nirS amoB nifH
HMA A. archeri Florida 0/1 0/1 0/1 0/1
A. tubulata Florida 6/6 5/5 0/1 0/1
Belize 4/5 3/4 0/1 0/1
I. strobilina Florida 0/2 0/2 0/2 0/2
V. gigantea Florida 4/6 0/2 0/1 0/1
Belize 2/2 0/2 0/1 0/1
V. reiswigi Florida 1/2 0/2 0/1 0/1
Belize 2/4 1/4 0/1 0/1
X. muta Florida 5/5 0/5 0/1 0/1
Belize 4/5 0/5 0/1 0/1
LMA C. plicifera Florida 0/3 0/2 0/1 0/1
Belize 0/3 0/2 0/1 0/1
C. vaginalis Florida 0/3 0/2 0/1 0/1
Belize 0/3 0/2 0/1 0/1
M. laxissima Florida 0/3 0/2 0/1 0/1
Belize 0/3 0/2 0/1 0/1
N. digitalis Florida 0/3 0/2 0/1 0/1
Belize 0/3 0/2 0/1 0/1