Table 5 PCR-based screening of nitrogen cycling genes in 10 sponge species from 2 locations, showing the number of samples testing positive (left value) and the total number of samples tested (right value) for 4 functional genes. Amplifications for amoB and nifH genes were repeated in triplicate for each sample
Category | Species | Location | Function gene PCR assays | |||
|---|---|---|---|---|---|---|
amoA | nirS | amoB | nifH | |||
HMA | A. archeri | Florida | 0/1 | 0/1 | 0/1 | 0/1 |
A. tubulata | Florida | 6/6 | 5/5 | 0/1 | 0/1 | |
Belize | 4/5 | 3/4 | 0/1 | 0/1 | ||
I. strobilina | Florida | 0/2 | 0/2 | 0/2 | 0/2 | |
V. gigantea | Florida | 4/6 | 0/2 | 0/1 | 0/1 | |
Belize | 2/2 | 0/2 | 0/1 | 0/1 | ||
V. reiswigi | Florida | 1/2 | 0/2 | 0/1 | 0/1 | |
Belize | 2/4 | 1/4 | 0/1 | 0/1 | ||
X. muta | Florida | 5/5 | 0/5 | 0/1 | 0/1 | |
Belize | 4/5 | 0/5 | 0/1 | 0/1 | ||
LMA | C. plicifera | Florida | 0/3 | 0/2 | 0/1 | 0/1 |
Belize | 0/3 | 0/2 | 0/1 | 0/1 | ||
C. vaginalis | Florida | 0/3 | 0/2 | 0/1 | 0/1 | |
Belize | 0/3 | 0/2 | 0/1 | 0/1 | ||
M. laxissima | Florida | 0/3 | 0/2 | 0/1 | 0/1 | |
Belize | 0/3 | 0/2 | 0/1 | 0/1 | ||
N. digitalis | Florida | 0/3 | 0/2 | 0/1 | 0/1 | |
Belize | 0/3 | 0/2 | 0/1 | 0/1 | ||