Fig. 1

Animal work. a Outline of experimental procedures. Time in weeks is shown along the top of the figure. Animals arrived in two batches, 3 weeks apart, at age 7 weeks, and after a week-long acclimatisation period, underwent a 2-stage subtotal nephrectomy or sham procedure. Eight weeks after the second stage of this procedure, after a 24-h urine collection, they were sacrificed and samples of serum and caecal fluid collected. b Weight at time of sacrifice (p = 0.033 for treatment, p = 0.586 for batch, by 2-way ANOVA). c 24 h urine volumes immediately before sacrifice (p = 0.0009 for treatment, p = 0.256 for batch, by 2-way ANOVA). d Serum urea at time of sacrifice (p < 0.0001 for treatment, p = 0.392 for batch, by 2-way ANOVA). e Serum creatinine at the time of sacrifice (p < 0.0001 for treatment, p = 0.645 for batch, by 2-way ANOVA)