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Table 1 Selected MAGs assembled from 13C2 glycine betaine stable isotope labelling microcosms

From: A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments

Sampleǂ Bin Taxonomy assignment by RAST Length (bp) Completeness (%) Contamination (%) grdH mttB
Class Species
Heavy_R1 4 Clostridia Thermincola sp. JR 2.69E+06 94.5 2.3 +  
Light_R1 23 Clostridia Thermincola sp. JR 2.88E+06 98.7 0.8 +  
Heavy_R1 5 Clostridia Clostridium thermocellum 2.80E+06 73.5 4.7   
Light_R3 10 Clostridia Desulfotomaculum reducens 5.28E+06 98.7 8.1 +  
Heavy_R3 21 Clostridia Thermincola sp. JR 1.77E+06 78.5 3.0   
Heavy_R3 8 Clostridia Desulfotomaculum acetoxidans 2.99E+06 97.8 2.5   
Light_R3 22 Clostridia Thermincola sp. JR 2.13E+06 84.7 2.3   
Light_R2 5 Clostridia Thermincola sp. JR 2.38E+06 76.0 1.3 +  
Heavy_R2 3 Clostridia Moorella thermoacetica 2.81E+06 87.5 4.9   
Heavy_R2 21 Deltaproteobacteria Desulfobacterium autotrophicum 6.49E+06 94.1 7.0   
Heavy_R3 22 Deltaproteobacteria Desulfobacterium autotrophicum 6.62E+06 98.5 4.8   
Heavy_R3 5 Deltaproteobacteria Desulfobacterium autotrophicum 5.81E+06 93.4 9.8   
Light_R3 26 Deltaproteobacteria Desulfuromonas acetoxidans 2.61E+06 83.0 9.4   
Light_R1 27 Deltaproteobacteria Desulfuromonas acetoxidans 2.11E+06 75.4 8.4   
Light_R1 10 Deltaproteobacteria Desulfuromonas acetoxidans 3.62E+06 96.6 6.8   
Light_R3 12 Deltaproteobacteria Desulfuromonas acetoxidans 2.72E+06 93.0 2.2   
Heavy_R3 28 Deltaproteobacteria Desulfuromonas acetoxidans 2.41E+06 83.6 2.2   
Light_R2 9 Deltaproteobacteria Sorangium cellulosum 1.48E+06 73.0 3.4   
Light_R3 14 Gammaproteobacteria Cellvibrio japonicus 1.66E+06 72.9 2.4   
Heavy_R2 6 Thermomicrobia Thermobaculum terrenum 1.81E+06 79.9 3.3   
Heavy_R1 6 Euryarchaeota Methanosarcina barkeri 2.35E+06 75.8 1.0   +
Heavy_R3 2 Euryarchaeota Methanosarcina barkeri 2.21E+06 74.3 0.0   +
Light_R1 18 Euryarchaeota Methanosarcina barkeri 2.16E+06 74.6 1.3   +
Heavy_R2 7 Euryarchaeota Methanococcoides burtonii 1.56E+06 74.0 1.3   +
  1. Only MAGs with > 70% completeness and < 10% contamination are shown. gdH encodes for a glycine betaine reductase, and mttB encodes for a pyrrolysine-containing trimethylamine methyltransferase
  2. ǂ‘heavy’ and ‘light’ DNA fractions from three independent biological replicates (R1, R2, R3) were sequenced and assembled