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Fig. 2 | Microbiome

Fig. 2

From: A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments

Fig. 2

Comparative genomics analysis showing the average nucleotide identity (ANI) and average amino acid identity (AAI) values of this novel clostridial bacterium (bin 4, see Table 1) compared to the genome-sequenced bacteria in the order of Clostridiales [14]. The tree was constructed using 16S rRNA genes and phylogenetic analysis was conducted in MEGA7 [15]. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches. The scale bar represents the number of substitutions per nucleotide. ANI and AAI values were calculated using the corresponding calculators as described in Rodriguez and Konstantinidis [16]. NA indicates that the ANI values are too close to the detection limit, and thus, no reliable values were returned

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