Table 1 Sequences, annealing temperatures, fragment length, and sources of the implemented qPCR primers. The primers for Vishniacozyma and Plectospaerella were designed with deposited sequences (accession numbers provided) in the NCBI database and the Primer-BLAST tool [53]
From: Microbiome-driven identification of microbial indicators for postharvest diseases of sugar beets
Taxonomic group | Forward primer | Reverse primer | Length (bp) | Annealing temp (°C) | Reference/source |
|---|---|---|---|---|---|
Vishniacozyma | CGCATCGATGAAGAACGCAG | AAAACCCAAGTGGGGTGAGG | 151 | 64.6 | NR_073260.1, this study |
Plectospaerella | ATCTCTTGGCTCCAGCATCG | GATACTGGAAGGCGCCATGT | 112 | 65 | GU724980.1, this study |
Candida | TCTAACGTCTATGCGAGTG | ATACCCAAATTCGACGATCG | 244 | 59.4 | [54] |
Fusarium | CAACTCCCAAACCCCTGTGA | GCGACGATTACCAGTAACGA | 398 | 58 | [55] |
Lactobacillus | GCAGCAGTAGGGAATCTTCCA | GCATTYCACCGCTACACATG | 342 | 62.1 | [56] |
Penicillium | ATGAAATCCTCCCTGTGGGTTAGT | GAAGGATAATTTCCGGGGTAGTCATT | 92 | 65 | [57] |