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Table 1 The sampling locations and characteristics of the samples collected from five DWDSs (A–E). Water samples in each location were collected in four seasons: winter (January to February), spring (March to May), summer (August to September), and autumn (October to December) in two consecutive weeks

From: Active eukaryotes in drinking water distribution systems of ground and surface waterworks

DWDS

Water source

Treatment process

Disinfection

Location

Distance (km)

Sample type*

Sample count

Average total chlorine (mg/l)

Range

Average temp. (°C)

Range

A

Artificial groundwater

Aeration, lime stabilization, flocculation, clarification, addition of sulphuric acid, sand filtration

No disinfection

1

2

Cold

8

–

–

12.7

8–18

2

8

Cold

8

–

–

10.6

7–14

‘’

‘’

Hot

8

–

–

53.8

51–56

3

11

Cold

8

–

–

9.2

6–14

i

na

WM

2

–

–

na

na

B

Artificial groundwater

Aeration, lime stabilization, flocculation, clarification, sand filtration

No disinfection

1

1

Cold

8

–

–

9.6

7–12

2

3

Cold

8

–

–

10.4

8–13

‘’

‘’

Hot

8

–

–

57.7

53–62

3

8

Cold

8

–

–

8.9

6–13

i

na

WM

2

–

–

na

na

C

Surface water

Ferric sulfate coagulation, flotation, sand filtration, activated carbon filtration

UV-light, ClO2, Cl2

1

2

Cold

7

0.5

0.4–0.6

8.9

2–20

2

8

Cold

8

0.3

0.2–0.5

11.8

5–19

‘’

‘’

Hot

8

na

na

50.6

43–54

3

20

Cold

7

0.2

0.1–0.4

10.1

6–16

i

na

WM

2

na

na

na

na

D

Surface water

Ferric sulfate coagulation, clarification, sand filtration, ozonisation, activated carbon filtration

UV-light, NH2, Cl2

1

5

Cold

8

0.1

< 0.03–0.2

10.3

7–15

2

14

Cold

8

0.1

0.03–0.4

10.5

6–17

‘’

‘’

Hot

8

na

na

54.6

51–58

‘’

‘’

Pipe

3

na

na

na

na

3

19

Cold

8

0.1

< 0.03–0.3

8.1

5–13

i

na

WM

1

na

na

na

na

E

Groundwater

Aeration, limestone filtration

UV-light, NaOCl

1

9

Cold

8

0.2

0.1–0.4

6.2

5–9

2

26

Cold

9

0.1

0.1–0.2

7.0

5–11

‘’

‘’

Hot

8

na

na

54.1

49–59

‘’

‘’

Pipe

3

na

na

na

na

3

36

Cold

8

0.2

0.1–0.5

6.0

4–10

i

na

WM

3

na

na

na

na

  1. *100 l tap water samples (Cold) and 100 l of water from the warm water system of the building (Hot); water meter biofilm, i.e., loose deposit (WM), pipeline biofilm (Pipe). Pipe includes three replicates from a same pipeline. WM ages (years): DWDS A (14, 15); DWDS B (7, 10); DWDS C (5, 9); DWDS D (8); DWDS E (15, 17, 19). Additional 100 l cold water samples collected with water meter biofilms: N(DWDS A) = 2, N(DWDS B) = 2, and N(DWDS C) = 1. na not applicable