Competitive interaction with keystone taxa induced negative priming under biochar amendments

Microbiome

Table 1 The keystone taxa in the bacterial and fungal networks under non-amended and biochar-amended treatments

Network	OTU ID	Module	Degree	Cluster coefficient	Abundance (%)^a		Phylum/class	Affiliation	Z^b	P^b	Diversity	Carbohydrate	qCO₂
Network	OTU ID	Module	Degree	Cluster coefficient	Field	SIP incubation	Phylum/class	Affiliation	Z^b	P^b	Diversity	Carbohydrate	qCO₂
Bacteria
Non-amended	OTU2131	Module I	8	0.22	0.25	1.15	Alphaproteobacteria	Sphingomonas	2.67	0.22	− 0.357	0.627*	0.602*
Biochar-amended	OTU717	Module I	8	0.23	0.20	12.39	Actinobacteria	Arthrobacter	3.12	0	0.798***	− 0.879***	− 0.756**
	OTU316	Module II	9	0.29	0.09	0.18	Gemmatimonadetes	Gemmatimonadaceae	2.95	0.19	0.617*	− 0.841***	− 0.789***
Fungi
Non-amended	OTU148	Module II	6	0.35	0.05	5.44	Eurotiomycetes	Aspergillus	2.53	0	− 0.479	0.763**	0.762**
Biochar-amended	OTU430	Module II	7	0.57	0.26	0.09	Sordariomycetes	Chaetomium	2.57	0	0.832***	− 0.722**	− 0.654**
	OTU516	Module II	7	0.62	0.14	7.14	Eurotiomycetes	Penicillium	2.57	0	0.609*	− 0.722**	− 0.642**

qCO₂, soil metabolic quotient. *P < 0.05; **P < 0.01; ***P < 0.001.
^aAbundance of keystone species in the field experiment and stable isotope probing (SIP) microcosms
^bThe topological role of each node is determined based on two properties: the within-module connectivity Z and the among-module connectivity P

ISSN: 2049-2618