Metagenomic analysis of isolation methods of a targeted microbe, Campylobacter jejuni, from chicken feces with high microbial contamination

Table 1 Culture-dependent isolation results of Campylobacter jejuni and competing microbes in each procedure

Enrichment type		No enrichment		Bolton broth						Preston broth
Ratio		−		1:10¹		1:10²		1:10³		1:10¹		1:10²		1:10³
Selective media		mCCDA	Preston agar	mCCDA	Preston agar	mCCDA	Preston agar	mCCDA	Preston agar	mCCDA	Preston agar	mCCDA	Preston agar	mCCDA	Preston agar
Target and competing microbes	C. jejuni	48.6% (17/35)	54.3% (19/35)	−	−	−	−	−	−	−	−	31.4% (11/35)^ac	−	97.1% (34/35)^abc	5.7% (2/35)
	ESBLs producing E. coli	48.6% (17/35)^a	8.6% (3/35)	80.0% (28/35)^ac	−	97.1% (34/35)^ac	2.9% (1/35)	82.9% (29/35)^ac	54.3% (19/35)^bc	48.6% (17/35)^a	−	42.9% (15/35)^a	−	11.4% (4/35)^b	−
	P. mirabilis	42.9% (15/35)	88.6% (31/35)^a	45.7% (16/35)	94.3% (33/35)^a	71.4% (24/35)	91.4% (32/35)	62.9% (22/35)^c	82.9% (29/35)	65.7% (23/35)	94.3% (33/35)	54.3% (19/35)	80.0% (28/35)^a	28.6% (10/35)^b	97.1% (34/35) ^a
	Enterococcus spp.	22.9% (8/35)^a	2.9% (1/35)	−	−	−	−	−	−	20.0% (7/35)^ac	2.9% (1/35)	31.4% (11/35)^ac	2.9% (1/35)	17.1% (6/35)	8.6% (3/35)

A total of 14 procedures were applied for all 35 fecal samples, which were combinations of different enrichment type (without enrichment process or enriched in Bolton broth or Preston broth), ratio of sample-to-enrichment broth (1:10¹, 1:10², or 1:10³), and selective media (mCCDA or Preston agar)
^aSignificant differences according to selective media (enriched in the same enrichment broth and the same ratio of sample-to-broth)
^bSignificant differences according to the ratio of sample-to-broth (enriched in the same enrichment broth and same selective media)
^cSignificant differences according to the type of enrichment broth (enriched in the same ratio of sample-to-broth and the same type of selective media), −, negative result (0%).

ISSN: 2049-2618