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Fig. 8 | Microbiome

Fig. 8

From: Metagenomic analysis of isolation methods of a targeted microbe, Campylobacter jejuni, from chicken feces with high microbial contamination

Fig. 8

Quantitative PCR of C. jejuni applied to all 54 samples. a Log CFU/ml of C. jejuni in each procedure (mean ± SEM) inferred from the standard curve and b correlation of metagenomics data and quantitative polymerase chain reaction (qPCR) results. Significance was determined by Mann-Whitney U test. The amount of C. jejuni was significantly higher in fecal samples in 103-Preston broth, 1.0 × 1010.2 ± 0.2, followed by 102-Preston broth (1.0 × 107.3 ± 1.3) and 103-Bolton broth (1.0 × 103.9 ± 1.5). In addition, the cycle threshold (Ct) values from qPCR results and the number of Campylobacter reads from metagenomics results had a high correlation. *p < 0.05, **p < 0.01

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Microbiome

ISSN: 2049-2618

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