Phylogenetic tree of Circo-like viruses. Viral family-specific protein marker Rep was used to construct phylogenetic trees from mangrove virome contigs and reference sequences for Circo-like viruses. Environmental viral sequences amplified from Reclaimed Water (RW), Chesapeake Bay (CB), Sargasso Sea (SAR) or British Columbia (BBC) were considered as additional references [39, 40], as well as the Rep genes from CHIV  and two diatoms viruses (Chaetoceros viruses). Reference sequences are coloured in black, whereas virome contigs are indicated with different colours in the tree. The tree was bootstrapped with 100 subreplicates based on maximum-likelihood methods, and nodes with confidence levels higher than 30% are flagged with circles. The scale bar represents one amino acid substitution per site.