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Table 1 Summary of results

From: Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data

 

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

 

DIAMOND+MEGAN

Unicycler

Total

Aligned

Average

CheckM

Prokka

 

taxonomic bin

contigs

(Mb)

(Mb)

coverage

Complete.

contam.

rRNA

tRNA

CDS

High-quality draft genomes:

          

B1

Bacteroidetes bacterium OLB12

1

4.2

3.5

57.3

95%

0.1%

6

39

4,163

B2

Candidatus Accumulibacter SK-02

1

5.2

4.1

384.2

94%

0.6%

4

53

4,915

B3

Chlamydiia (class)

1

2.8

1.8

48.8

94%

2%

6

39

3,387

B4

Gammaproteobacteria (class)

43

4.7

3.0

 

93%

2%

6

52

4,833

 

-Longest contig

 

2.7

1.6

25.1

93%

0.2%

3

40

3,359

B5

Bacteroidetes bacterium OLB8

1

3.8

3.0

52.1

93%

1%

6

37

3,394

B6

Rhodospirillales (order)

1

4.4

3.0

29.5

92%

0.5%

3

47

4,015

B7

Chlorobi bacterium OLB5

1

3.5

2.5

38.7

88%

1%

3

41

4,131

Medium quality draft genomes:

          

B8

Thauera (genus)

25

4.6

4.0

 

89%

4%

12

64

4,040

 

-Longest contig

 

0.8

0.7

32.7

14%

0%

0

5

672

B9

Sphingobacteriales bacterium 44-15

59

3.2

2.8

 

76%

1%

2

17

2,953

 

-Longest contig

 

0.2

0.1

10.2

0%

0%

0

0

172

B10

Bacteroidetes (phylum)

43

3.9

2.6

 

72%

7%

1

12

1,997

 

-Longest contig

 

1.2

0.8

14.1

32%

0%

0

3

807

B11

Candidatus Contendobacter B J11

39

2.5

2.0

 

59%

9%

2

37

2,668

 

-Longest contig

 

0.3

0.3

15.4

19%

0%

0

7

295

Low quality draft genomes:

          

B12

Betaproteobacteria (class)

111

6.6

5.5

 

89%

79%

6

71

4,655

 

-Longest contig

 

0.4

0.3

37.1

10%

0%

0

1

372

B13

Nitrospira (genus)

34

4.2

3.7

 

83%

13%

0

6

563

 

-Longest contig

 

1.1

0.9

17.6

27%

0%

0

2

99

B14

Chloroflexi (phylum)

151

5.4

4.3

 

71%

29%

0

11

3,565

 

-Longest contig

 

0.2

0.2

13.3

8%

0%

0

1

86

  1. For all 14 taxonomic bins B1–B14 that CheckM deems ≥ 50% complete (a), and -in cases where the bin contains more than one contig- also for the longest contig, in descending order of assembly quality, we report (b) the number of contigs produced by Unicycler, (c) the total number of bases, (d) the number of bases aligned by DIAMOND to some protein reference, (e) the average coverage by long reads (based on the longest contig), (f) the %-completeness and (g) %-contamination reported by CheckM, and (h)–(j), the number of rRNA, tRNA and coding sequences reported by Prokka, respectively