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Fig. 2 | Microbiome

Fig. 2

From: Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data

Fig. 2

Analysis. a Long-read analysis pipeline shown from left to right. MinION sequencing produces a set of reads. These are assembled into contigs using Unicycler and aligned against the NCBI-nr database using DIAMOND. The contigs and alignments are processed by MEGAN so as to perform taxonomic binning and also to produce frame-shift-corrected contigs. These are analyzed using CheckM and annotated using Prokka. The duration of each step is shown in wall-clock hours. MEGAN analysis took less than 10 min. b Frame-shift correction: in frame-shift alignments, forward slashes, and backward slashes indicate a frame decrease, or increase, by one, respectively. Correction is performed by inserting one or two unspecified nucleotides into the sequence, respectively

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