Fig. 7From: Fecal metatranscriptomics of macaques with idiopathic chronic diarrhea reveals altered mucin degradation and fucose utilizationStaining and examination of macaque intestines for mannose and fucose indicates the altered presence of fucose in ICD. Proximal colon sections were stained with 10 μg/mL FITC-labeled Ulex europaeus agglutinin 1 or concanavalin A to visualize fucose and mannose, respectively, and counterstained with DAPI. Slides were imaged with a confocal microscope at the same FITC channel voltage setting for each lectin. Five micrographs were analyzed from each animal to calculate a background-corrected crypt mean pixel intensity as described under the “Methods” section. a Representative micrographs showing sections from control and ICD animals stained for mannose or fucose. Nuclei stained with DAPI are shown in blue, and mucin sugars are displayed in green. b Average crypt pixel intensity of mannose and fucose signal. ***p = 0.008, two-tailed t test; n = 5 for control, n = 6 for ICD animalsBack to article page