Fig. 1
From: Systemic translocation of Staphylococcus drives autoantibody production in HIV disease
Autoantibodies generated after seasonal influenza vaccination in HIV-infected individuals. ELISA and autoantibody array were performed using plasma samples on D0 and D14 post-vaccination. Detection of plasma IgG ANA (a) and anti-dsDNA (b) autoantibodies from healthy (n = 16) and HIV+ (n = 26) subjects. c Detection of IgG autoantibodies by protein array containing 125 self-antigens from healthy (n = 16), HIV+ low ANA (n = 14), and HIV+ high ANA (n = 12) subjects. d Autoreactivity and polyreactivity of four anti-dsDNA mAbs (left margin, clone number, and CDR3 sequence) and one anti-influenza HA mAb (V21-HA-4) as a control by ELISA. (E) Polyreactivity of anti-dsDNA mAb and anti-influenza HA mAb against cellular nuclear antigens (Hep-2 cells) by immunofluorescent microscopy. Original magnification, × 40. Non-parametric Mann-Whitney U tests