Fig. 2
Functional features of family S24-7. a Quality plot of the genome sequences used for analysis. Assemblies generated in the present study (isolates and metagenomic species) were considered if the marker gene completeness minus contamination was ≥ 80% [45]. Other reconstructed genomes are from two studies previously published [44, 46]. b Non-supervised clustering based on glycoside hydrolases (GH) occurrences across all 153 genomes available (numbered in increments of 10 on the right-hand side) as performed previously [44]. Already published entries are labeled in blue (“Homeothermaceae,” n = 30) or violet (UBA, n = 37) letters. Those from the present study are in black (MGS, n = 59), gray (short-term isolates, n = 21), or gold (cultured strains; n = 5 novel and 1 type species previously published). For the isolates in gray, names in brackets indicate their facility/vendor of origin (HZI, Helmholtz Center for Infection Research, Braunschweig, Germany; NCI, National Cancer Institute, Maryland, USA; Harlan; Janvier). GH categories (labels on top) considered discriminative between the different functional guilds (a-glucan, host or plant glycans) are colored accordingly (green, orange, and brown, respectively). c Multidimensional plotting of family S24-7 members and those from neighboring families based on KEGG orthology (KO). d Family-specific functions. The plots depict the prevalence (%) of single KOs across the different genome categories (top labels with numbers in brackets). The twelve KOs in violet (top) are specific for the S24-7 family, the seven bluish KOs (bottom) for the members of other families (see panel c) within the order Bacteroidales. KO definition are as follows (from top to bottom): K01577, oxalyl-CoA decarboxylase [EC:4.1.1.8]; K07749, formyl-CoA transferase [EC:2.8.3.16]; K01821, 4-oxalocrotonate tautomerase [EC:5.3.2.6]; K07088, uncharacterized protein; K01058, phospholipase A1/A2 [EC:3.1.1.32 3.1.1.4]; K07054, uncharacterized protein; K11921, family transcriptional regulator, cyn operon transcriptional activator; K21993, formate transporter fdhC; K07126, uncharacterized protein; K06143, inner membrane protein creD; K00283, glycine dehydrogenase subunit 2 [EC:1.4.4.2]; K00282, glycine dehydrogenase subunit 1 [EC:1.4.4.2]; K03685, ribonuclease III [EC:3.1.26.3]; K03284, magnesium transporter; K03575, A/G-specific adenine glycosylase [EC:3.2.2.31]; K01938, formate-tetrahydrofolate ligase [EC:6.3.4.3]; K13993, HSP20 family protein; K00656, formate C-acetyltransferase [EC:2.3.1.54]