Fig. 4
From: Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
a Amino acid frequency plot of the P1 and P1′ sites as detected from peptide sequencing of LC-MS/MS data against the human genome. The size of the amino acid indicates the percent difference in abundance from others normalized to the relative abundance of each amino acid in the human peptidome. Those above the double line are significantly more abundant, while those below the line are significantly less abundant. Amino acids are colored based on their physiochemical properties (blue = positive charge, red = negative charge, black = hydrophobic, green = hydrophillic). b Protease activity in sputum samples using Ala-Ala-Pro-Val-AMC and Ala-Ala-Pro-Phe-AMC. The velocity of the reaction was measured as a change in fluorescent intensity per second. Activity from the two metabolomic clusters is shown as notch plots. c Notch plots of the normalized abundance of human neutrophil peptides in sputum samples from the two metabolomic clusters