Fig. 2

Effects of chlorpyrifos administration on fasting glucose, fasting insulin, glucose tolerance, and insulin sensitivity in C57bl/6 (a–e) and CD-1 (ICR) mice (f–j). Mice were deprived of food for 12 h and 6 h to determine fasting glucose (a, f) and insulin (b, g). HOMA-IR index was calculated based on fasting glucose and insulin (c, h). Mice were fasted for 12 h, and an oral glucose tolerance test (d, i) was performed. Mice were fasted for 6 h, and an insulin tolerance test (e, j) was performed (n = 8). Data are expressed as the mean ± SEM. *P < 0.05 vs. NFD group; ^#P < 0.05 vs. HFD group. NFD normal-fat diet, NCPF normal-fat diet + chlorpyrifos, HFD high-fat diet, HCPF high-fat diet + chlorpyrifos, HOMA-IR homeostasis model assessment of insulin resistance, OGTT oral glucose tolerance test, ITT insulin tolerance test