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Fig. 5 | Microbiome

Fig. 5

From: Taxonomic profiling and populational patterns of bacterial bile salt hydrolase (BSH) genes based on worldwide human gut microbiome

Fig. 5

Molecular docking and enzyme activity comparison. The results of molecular docking and kinetic reaction of eight BSH phylotypes with a glycocholic acid (GCA), b glycochenodeoxycholic acid (GCDCA), c glycine deoxycholic acid (GDCA), d taurocholic acid (TCA), e taurochenodeoxycholic acid (TCDCA), f taurodeoxycholic acid (TDCA), and g tauroursodeoxycholic acid (TUDCA). The left panel shows the binding energy (BD energy) of each BSH, and molecular docking predicted binding models of BSH-T3 with different bile acids. The middle panel showed the eight BSHs in the kinetic reaction with different bile acids. The right panel compares the deconjugation (the proportion of product bile acid of specific substrate) in light green bar when the substrate concentration is 0.1 mM, and specific activity (the activity of an enzyme per milligram of total protein, using the highest enzyme activity as 100%) of eight BSHs with different bile salts (final concentration of all bile salts was 20 mM) in light purple bar when the substrate concentration is 20 mM. Data were analyzed with student’s t test corrected by false discovery rate. *p < 0.05, **p < 0.01, and ***p < 0.05, versus the BSH which shown the highest enzyme activity for the specific bile salt, i.e., BSH-T3 showed the highest enzyme activity when the substrates were TCDCA and TDCA, for TCA, it is BSH-T1. Details are shown in Additional file 2: Table S7–S9

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