Fig. 5
From: Design and application of a novel two-amplicon approach for defining eukaryotic microbiota
Associations between eukaryotic and bacterial microbiota. a Co-occurrence analysis between eukaryotic and bacterial microbiota. Shown is a heatmap of Hamming distances between genera represented by ≥ 5 reads in 20–80% of samples (n = 44). Red indicates a high co-occurrence score. The detail highlights bacterial genera clustering with Blastocystis, and the yellow line denotes a cluster of Enterobacteriaceae. b Association of bacterial composition with the presence of Blastocystis. Plots were generated with ALDEx2 and show OTU fold change versus median abundance (left) and OTU fold change between versus within conditions (right). Red points indicate OTUs with significant compositional changes (Wilcoxon rank test). c sPLS-DA analysis showing separation of patient samples based on the abundance of bacterial genera in Blastocystis positive and negative patients. d Mean relative sequence abundances of a subset of bacterial genera, in Blastocystis positive and negative patients. Error bars represent standard error. e Comparison of alpha diversity of samples positive and negative for Blastocystis (15; 29), Cryptosporidium (12; 32), and Giardia (14; 26), measured using the Shannon index. The presence of Blastocystis and Cryptosporidium were counted with a minimum of five reads. *p < 0.05, **p < 0.005