Fig. 2

Phylogenetic tree of IsoA sequences retrieved from enriched environmental samples. Sequences in black represent IsoA sequences from bona-fide isoprene degraders. Sequences obtained from clone libraries and amplicon sequencing of leaf samples are represented in green, soils in brown, freshwater environments in light blue and marine sediments in dark blue. Environments where a particular OTU is abundant are shown in brackets. Only OTUs with ≥ 2% abundance in at least one of the samples are represented. The tree was drawn in Mega7 [82] using the neighbour-joining method and the Jones-Taylor-Thornton model. Scale bar indicates 0.05 substitutions per site. Bootstrap values ≥ 50% (based on 1000 replicates) are represented with dots at branch points