Fig. 4From: Long-term soil metal exposure impaired temporal variation in microbial metatranscriptomes and enriched active phagesDefinition, abundance, and phylogenetic composition of microbial temporal response groups (TRGs). Panel a shows the statistical definition and validation of the TGRs in each plot. OTUs with significant temporal response were extracted in each Cu plots (nbGLM, LRT, FDR-corrected p < 0.05), and grouped using hierarchical clustering. Validation of TRGs was done with a Between Group Analysis (BGA) and Monte-Carlo simulations with 100,000 group permutations using all OTUs to reinforce randomization power. Panel b shows the relative abundance of TRGs in each plot according to sampling time, while panel c displays the phylogenetic composition of TRGsBack to article page