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Fig. 1 | Microbiome

Fig. 1

From: Long-term soil metal exposure impaired temporal variation in microbial metatranscriptomes and enriched active phages

Fig. 1

Redundancy analysis (RDA) on 16S rRNA transcript amplicon profiles (cDNA, n = 54, panel a) and metatranscriptomes (mRNA, n = 26, panel b). Ordination plots were constrained using a model with explanatory variables (gray arrows) using Bray-Curtis dissimilarly index (R package vegan, capscale function, adjusted r2 from model tested with 10,000 permutations). Explanatory variables include substrate respiration (MicroResp, D(+) galactose (GAL), l-malic acid (MAL), gamma amino butyric acid (GABI), n-acetyl glucosamine (AGL), D(+) Glucose (GLU), Alpha Ketogluterate (AKET) and Citric Acid (CIT), PLFA fractions (G+: Gram positive, G-: Gram negative, Acti: Actinobacteria, Fun: Fungi), soil pH and moisture, bioavailable Cu (BioCu), proportion of phage mRNA signatures, and phylogenetic relatedness (− 1* MPD Z-score). Plots display the first and second constrained components with percentage of explained variance in datasets by the model. The colored plot names are indicating their centroid location as discriminant factors in the model

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