Fig. 1

IgG cell sorting and 16S rRNA gene sequencing identify microbes collected from pediatric IBD patient ileal wishes. a Sequence of procedures for the identification of IgG-bound pathobiont microbes. b Representative of cell sorting of patient sample #81 by BD FACSAria III with a shift in population of IgG+ bacteria (33.3%). The Q1 and Q2 quadrants comprise PI positive/IgG negative (IgG−) and PI positive/IgG positive (IgG+), respectively. c Representative of image flow cytometry with shift in population of IgG+ bacteria. The Amnis ImagestreamX mkII was used for acquisition of image cytometry, performed at × 60 magnification. Gating compensation was performed using individual stained (DAPI, EUB338, or anti-IgG) sample controls. Analysis was performed using IDEAS Software version 6.1