TY - JOUR AU - Singh, Nitin Kumar AU - Wood, Jason M. AU - Karouia, Fathi AU - Venkateswaran, Kasthuri PY - 2018 DA - 2018/11/13 TI - Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces JO - Microbiome SP - 204 VL - 6 IS - 1 AB - The International Space Station (ISS) is an ideal test bed for studying the effects of microbial persistence and succession on a closed system during long space flight. Culture-based analyses, targeted gene-based amplicon sequencing (bacteriome, mycobiome, and resistome), and shotgun metagenomics approaches have previously been performed on ISS environmental sample sets using whole genome amplification (WGA). However, this is the first study reporting on the metagenomes sampled from ISS environmental surfaces without the use of WGA. Metagenome sequences generated from eight defined ISS environmental locations in three consecutive flights were analyzed to assess the succession and persistence of microbial communities, their antimicrobial resistance (AMR) profiles, and virulence properties. Metagenomic sequences were produced from the samples treated with propidium monoazide (PMA) to measure intact microorganisms. SN - 2049-2618 UR - https://doi.org/10.1186/s40168-018-0585-2 DO - 10.1186/s40168-018-0585-2 ID - Singh2018 ER -