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Fig. 3 | Microbiome

Fig. 3

From: Hidden in plain sight—highly abundant and diverse planktonic freshwater Chloroflexi

Fig. 3

Spatiotemporal distribution and cell shape of different Chloroflexi lineages based on CARD-FISH analysis. Seasonal dynamics and vertical stratification of different Chloroflexi lineages according to CARD-FISH analysis in a Lake Zurich at five sampling time points and b Řimov Reservoir at four sampling time points during the year 2015. Stacked bars show the percentage of DAPI-stained cells (top axis), and smooth lines show vertical profiles of water temperature, oxygen, and chlorophyll a (bottom axis). c Cell volume (μm3) of CARD-FISH-stained Chloroflexi CL500-11 (n = 277) and all prokaryotes (n = 3789) along the depth profile of Lake Zurich on November 3, 2015. Boxes show 5th and 95th percentile, and the vertical line represents the median. The percentage of CL500-11 abundance and biomass among prokaryotes of the same depth profile is shown at the right. d The abundance of Chloroflexi lineages in 65 m depth of Lake Biwa at four sampling times in 2016. e CARD-FISH images of different Chloroflexi lineages. An identical microscopic field is shown for each column, with the DAPI-stained cells in the top and bacteria stained by cluster-specific CARD-FISH probes of each cluster on the bottom. The scale is shown on the top right side of each DAPI-stained cell field

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