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Table 2 PCR primers targeting genes coding for antibiotic resistance

From: Longitudinal development of the dust microbiome in a newly opened Norwegian kindergarten

Gene Primer sequence PCR conditions Approximate amplicon size (bp)
mecA 5′-GTAGAAATGACTGAACGTCCGATAA-3′
5′-CCAATTCCACATTGTTTCGGTCTAA-3′
[51]
4 min 94 °C;30 cycles (45 s at 94 °C; 45 s at 50 °C; 60 s at 72 °C); 2 min at 72 °C 310
ermA 5′-GTTCAAGAACAATCAATACAGAG-3′
5′-GGATCAGGAAAAGGACATTTTAC-3′
[52]
5 min 94 °C; 30 cycles (30 s at 94 °C; 30 s at 52 °C, 60 s at 72 °C); 10 min 72 °C 421
aac(6′)-aph(2″) 5′-TTGGGAAGATGAAGTTTTTAGA-3′
5′-CCTTTACTCCAATAATTTGGCT-3′
[53]
3 min 95 °C; 35 cycles (20 s at 95 °C; 20 s at 57 °C; 30 s at 72 °C); 2 min 72 °C 174
vanA 5′-CATGAATAGAATAAAAGTTGCAATA-3′
5′-CCCCTTTAACGCTAATACGATCAA-3′
[54]
10 min 95 °C; 30 cycles (30 s at 94 °C; 30 s at 58 °C; 30 s at 72 °C); 10 min 72 °C 1030