Fig. 3
From: Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies
Variation as a function of input biomass. a Spearman correlation between expected 16S rRNA gene copies per microliter and calculated 16S rRNA gene copies per microliter. Values are log10-transformed. b Bray-Curtis distances (boxplots) and intraclass correlation coefficients (ICC) (line plots) stratified by dilution constant (e.g., 1:1 means stock, 1:1000 means diluted 1000-fold). ICC values are shown as means. c Heatmap of the coefficient of variation (CV) values for individual genera stratified by dilution constant. Grayscale cells on the left indicate mean relative abundances for each genus (also given as percentages in parentheses). d Shannon diversity as a function of dilution constant