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Table 2 Detection of P. knowlesi in blood at different concentrations following restriction enzyme treatment of DNA extracts

From: Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

  Pk reads/total number of reads Result
Pk parasites/μL R1 R2 R3 R1 R2 R3
61.2 \( \frac{54}{10994} \) \( \frac{383}{45566} \) \( \frac{208}{30568} \) + + +
50.4 \( \frac{44}{6160} \) \( \frac{180}{31360} \) \( \frac{305}{28068} \) + + +
39.6 \( \frac{31}{7562} \) \( \frac{231}{47096} \) \( \frac{44}{28514} \) + + +
28.8 \( \frac{26}{9030} \) \( \frac{84}{21680} \) \( \frac{95}{23942} \) + + +
18 \( \frac{18}{12074} \) \( \frac{24}{15722} \) \( \frac{62}{19654} \) + +
7.2 \( \frac{12}{20290} \) \( \frac{22}{19068} \) \( \frac{43}{63164} \) + +
0.72 \( \frac{2}{36190} \) \( \frac{2}{17220} \) \( \frac{6}{57076} \)
  1. Pk Plasmodium knowlesi, R replicate, + positive, negative
  2. Note: The read counts listed here represent the values obtained after trimming and filtering