
Pk reads/total number of reads

Result


Pk parasites/μL

R1

R2

R3

R1

R2

R3


61.2

\( \frac{54}{10994} \)

\( \frac{383}{45566} \)

\( \frac{208}{30568} \)

+

+

+

50.4

\( \frac{44}{6160} \)

\( \frac{180}{31360} \)

\( \frac{305}{28068} \)

+

+

+

39.6

\( \frac{31}{7562} \)

\( \frac{231}{47096} \)

\( \frac{44}{28514} \)

+

+

+

28.8

\( \frac{26}{9030} \)

\( \frac{84}{21680} \)

\( \frac{95}{23942} \)

+

+

+

18

\( \frac{18}{12074} \)

\( \frac{24}{15722} \)

\( \frac{62}{19654} \)

–

+

+

7.2

\( \frac{12}{20290} \)

\( \frac{22}{19068} \)

\( \frac{43}{63164} \)

–

+

+

0.72

\( \frac{2}{36190} \)

\( \frac{2}{17220} \)

\( \frac{6}{57076} \)

–

–

–

 Pk Plasmodium knowlesi, R replicate, + positive, − negative
 Note: The read counts listed here represent the values obtained after trimming and filtering