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Table 1 Host, source, original identification method and GenBank accession number of samples used in this study

From: Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Parasite Sample type Host Species identification diagnostic method/s DNA extraction method Source GenBank accession no.
Babesia divergens EDTA blood Meriones unguiculatus Microscopy and PCR [35] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB AJ439713
Babesia duncani EDTA blood Meriones unguiculatus Microscopy and PCR [35] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB HQ289870
Babesia microti EDTA blood Homo sapiens Microscopy and PCR [35] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB AB243680
Brugia malayi EDTA blood Felis catus Microscopy Qiagen Blood Mini Kit Andrew Moorhead, FR3 AF036588
Cytauxzoon felis EDTA blood Felis catus Microscopy Qiagen Blood Mini Kit David Peterson, UGA CXZRR18S
Leishmania donovani subsp. donovani RPMI culture in EDTA blood Homo sapiens Microscopy and PCR [36] w/s Qiagen Blood Mini Kit Marcos de Almeida, CDC PDB X07773
Leishmania donovani subsp. infantum RPMI culture in EDTA blood Homo sapiens Microscopy and PCR [36] w/s Qiagen Blood Mini Kit Marcos de Almeida, CDC PDB GQ332359
Loa loa EDTA blood Homo sapiens Real time PCR [37] Boiled lysate Thomas Nutman, NIH DQ094173
Mansonella perstans EDTA blood Homo sapiens Microscopy Boiled lysate Thomas Nutman, NIH
Plasmodium falciparum EDTA blood Homo sapiens Microscopy and PCR [38] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB PFARGEA
Plasmodium knowlesi EDTA blood Macaca mulatta Microscopy Qiagen Blood Mini Kit Amy Kong, CDC Malaria Branch PFARRSSU
Plasmodium malariae EDTA blood Homo sapiens Microscopy and PCR [38] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB PFARGBAB
Plasmodium ovale EDTA blood Homo sapiens Microscopy and PCR [38] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB KF696369
Plasmodium vivax EDTA blood Homo sapiens Microscopy and PCR [38] w/s Qiagen Blood Mini Kit Henry Bishop, CDC PDB X13926
Tryapnosoma cruzi RPMI culture in EDTA blood Homo sapiens Microscopy Qiagen Blood Mini Kit Marcos de Almeida, CDC PDB AF239980
Trypanosoma brucei subsp. gambiense
Trypanosoma brucei subsp. rhodesiense HMI-9 culture in EDTA blood Homo sapiens Microscopy Qiagen Blood Mini Kit Stephen Hajduk, UGA AJ009142
Wuchereria bancrofti Clotted blood Homo sapiens Microscopy Qiagen Blood Mini Kit Patrick Lammie, CDC PDB AF227234
  1. w/s With Sanger sequencing of PCR product; CDC Centers for Disease Control and Prevention; PDB Parasitic Diseases Branch; UGA University of Georgia; NIH National Institutes of Health