Fig. 3

Enzyme digestion enhances sensitivity of detection for mixed parasite infections. Restriction enzyme digestion of mixed parasite infections in human blood yields a clear reduction in human 18S rRNA reads per thousand (left panel, greyscale diamonds) and a 2- to 15-fold increase in parasite reads per thousand (right panel, colored circles). Samples were normalized according to the reads per thousand for reads derived from human host and parasite separately, with the central dotted line reflective of a zero fold change, which marks the undigested samples before treatment with restriction