Fig. 1
From: High-resolution ISR amplicon sequencing reveals personalized oral microbiome
Higher diversity in the 16-23S intergenic spacer region (ISR) compared to the 16S V1-V3 among oral streptococci. Left: alignment of the 16S V1-V3 regions of common oral Streptococcus sp. shows alternating conserved and hypervariable regions. The limited amount of variation makes it difficult to distinguish between oral streptococci using 16S rRNA gene sequencing. Right: alignment of the ISR sequence of the same Streptococcus sp. show much higher variation, as evident from large gap regions, with the most prominent conserved region being the alanine tRNA gene