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Fig. 3 | Microbiome

Fig. 3

From: Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies

Fig. 3

Modification to pPNA sequence reduces contamination without altering estimates of diversity. a Asteraceae host plant species (6 species) exhibited a reduction in contamination when amplified with modified pPNAs to reflect the single nucleotide substitution in their chloroplast V4 sequence (F5,1 = 31.07, p = 0.003). In contrast, non-Asteraceae species (3 species) exhibited an increase in contamination when amplified with a modified pPNA representing a single nucleotide mismatch (ANOVA F2,1 = 23.42, p = 0.04). b pPNA type had no effect on estimated diversity within (ANOVA F7,1 = 1.29, p = 0.29) or c between root microbiomes (PERMANOVA pseudo-F = 1.70, p = 0.10). Note that with the exception of C. solstitialis, Fig. 3 includes different DNA samples amplified with either universal or Asteraceae-modified pPNA for each host plant species. See Additional file 1: Figure S1 for an ordination of the same DNA samples amplified with both pPNAs

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Microbiome

ISSN: 2049-2618

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