Fig. 2From: Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studiesOccurrence of sequence mismatch between the universal V4 pPNA and the plastid 16S V4 locus across 500 land plant species. a We queried NCBI GenBank accessions and found six independent occurrences of mismatch including five plant families (Juglandaceae, Melastomataceae, Vivianaceae, Asteraceae, and Cactaceae), and one genus from the Orobanchaceae (Epifagus). b Plant taxa of the phylogenetic tree in a are colored according to the particular nucleotide mismatch between the universal V4 pPNA and plastid 16S V4 locus. We note that Juglandaceae, Vivianaceae, and Cactaceae are represented with only a single taxon and whether or not the observed mismatch extends to other members of these families is unknownBack to article page